Coding
BFRM52H

Part:BBa_K1438001:Experience

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-06)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1438001

Group: Bilkent-UNAMBG, 2018

Author: Mehmet Ali Hoşkan

Summary: We have conjugated the BFR with sfGFP in order to observe the cells that are carrying our constructs easily. Our BFR construct was initially in a backbone with mCherry. We verified the sequence and then conjugated sfGFP with BFR instead of mCherry. The reason was sfGFP’s stronger fluorescent feature than mCherry’s.

T--Bilkent-UNAMBG--cont.png

Figure 1: BFR sfGFP conjugation plasmid carrying cells were observed to have sfGFP signal and verified with colony PCR.

T--Bilkent-UNAMBG--icc.png

Figure 2: Fluorescent image of BFR sfGFP conjugation plasmid containing cells.

User Reviews

UNIQdce4e41c30d1a09c-partinfo-00000000-QINU UNIQdce4e41c30d1a09c-partinfo-00000001-QINU