Coding
BFRM52H

Part:BBa_K1438001:Experience

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-06)


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Applications of BBa_K1438001

Group: Bilkent-UNAMBG, 2018

Author: Mehmet Ali Hoşkan

Summary: We have conjugated the BFR with sfGFP in order to observe the cells that are carrying our constructs easily. Our BFR construct was initially in a backbone with mCherry. We verified the sequence and then conjugated sfGFP with BFR instead of mCherry. The reason was sfGFP’s stronger fluorescent feature than mCherry’s.

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Figure 1: BFR sfGFP conjugation plasmid carrying cells were observed to have sfGFP signal and verified with colony PCR.

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Figure 2: Fluorescent image of BFR sfGFP conjugation plasmid containing cells.

User Reviews

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